{"product_id":"cst-5935s","title":"CST,  5935S, BRSK1 (D10F2) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying BRSK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BRSK1 (D10F2) Rabbit Monoclonal Antibody (CST #5935) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBRSK1 (D10F2) Rabbit Monoclonal Antibody detects endogenous levels of total BRSK1 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly485 of human BRSK1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nain- pecific inase (BRSK1; SAD-B) and ain- pecific inase 2 (BRSK2; SAD-A) are serine\/threonine kinases closely related to AMPK. LKB1 phosphorylates Thr189 in the T-loop of BRSK1 and Thr174 in the T-loop of BRSK2, resulting in activation of the kinases (1). BRSK1 localizes to synaptic vesicles in the hippocampus and cerebellum, together with the active zone proteins Bassoon and CAST, and BRSK1 phoshorylates the active zone protein RIM1 (2). An alternatively spliced from of BRSK1 displays unique activity during the cell cycle, phosphorylating Ser131 of γ-tubulin and controling centrosome duplication (3). Neuronal polarization, including axon formation, is fundamental for normal brain development. BRSK1 -\/- and BRSK2 -\/- mice have defects in neuronal polarity and impaired corticogenesis (4). Knockdown of BRSK1 and BRSK2 diminishes axonal growth (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBR serine\/threonine kinase 1; BR serine\/threonine-protein kinase 1; Brain-selective kinase 1; Brain-specific serine\/threonine-protein kinase 1; BRSK1; FLJ43009; hSAD1; KIAA1811; protein kinase SAD1A; SAD1; SAD1 homolog; SAD1 kinase; SADB; Serine\/threonine-protein kinase BRSK1; Serine\/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 90\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801009049769,"sku":"5935S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5935s","provider":"Iright","version":"1.0","type":"link"}