{"product_id":"cst-59921s","title":"CST,  59921S, CAPAM\/PCIF1 (E8B1B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PCIF1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CAPAM\/PCIF1 (E8B1B) Rabbit Monoclonal Antibody (CST #59921) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCAPAM\/PCIF1 (E8B1B) Rabbit Monoclonal Antibody recognizes endogenous levels of total CAPAM\/PCIF1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro229 of human CAPAM\/PCIF1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPhosphorylated CTD Interacting Factor 1 (PCIF1), also known as CAPAM, is a WW domain-containing protein that was initially discovered to interact with phosphorylated RNA Polymerase II, where it inhibits CTD phosphatase activity of SCP1 to negatively affect gene expression (1,2). CAPAM\/PCIF1 has shown to be the methyltransferase responsible for methylating adenosine at the second position of mRNAs, promoting their translation (3,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC20orf67; Cap-specific adenosine methyltransferase; CAPAM; hCAPAM; hPCIF1; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; PCIF1; PDX-1 C terminus-interacting factor 1; PDX1 C-terminal inhibiting factor 1; phosphorylated CTD interacting factor 1; Phosphorylated CTD-interacting factor 1; PPP1R121; Protein phosphatase 1 regulatory subunit 121; protein phosphatase 1, regulatory subunit 121\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 85\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801012228265,"sku":"59921S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-59921s","provider":"Iright","version":"1.0","type":"link"}