{"product_id":"cst-59948s","title":"CST,  59948S, mCherry (E5D8F) Feline Chimeric Monoclonal Antibody","description":"Monoclonal Antibody for studying mCherry bacteria. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, mCherry (E5D8F) Feline Chimeric Monoclonal Antibody (CST #59948) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nmCherry (E5D8F) Feline Chimeric Monoclonal Antibody detects mCherry-tagged proteins (either N-terminal tagged or C-terminal tagged) exogenously expressed in cells. Please note that the mCherry tags add approximately 28kDa to the molecular weight of the fusion protein.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis recombinant chimeric antibody is engineered from mCherry (E5D8F) Rabbit mAb #43590 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, mCherry (E5D8F) Rabbit mAb #43590, is produced by immunizing animals with a full-length recombinant mCherry protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. mCherry is a monomeric, fluorescent, photostable red dye that was isolated from and further engineered from the Discosoma sea anemone (1). Its peak fluorescent excitation is at 587 nm and emission 610 nm (1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nmCherry; MCherry fluorescent protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Feline chimeric IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800269836457,"sku":"59948S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-59948s","provider":"Iright","version":"1.0","type":"link"}