{"product_id":"cst-60049t","title":"CST,  60049T, CD33 (E6V7H) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CD33. Validated for Immunoprecipitation,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, CD33 (E6V7H) Rabbit Monoclonal Antibody (CST #60049) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 53391 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD33 (E6V7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD33 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CD33 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD33, a type I transmembrane protein, is a sialic acid-binding Ig-like lectin 3 (Siglec-3) of the Ig superfamily, and human CD33 binds preferentially to alpha-2, 6-linked sialic acid. Upon binding to its ligands, CD33 transduces inhibitory signaling through the immunoreceptor tyrosine-based inhibitory motif (ITIM) in its intracellular domain, inhibiting cellular functions such as phagocytosis. In addition, CD33 is also involved in other processes, such as adhesion (1-3). Due to its exclusive expression on hematopoietic cells, particularly the myeloid lineage and their progenitors, CD33 has been actively pursued as a therapeutic target against acute myeloid leukemia (AML) (4,5). CD33 may also be involved in Alzheimer's Disease (AD) (6-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD33; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec-3; SIGLEC3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 70-80\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800248144041,"sku":"60049T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-60049t","provider":"Iright","version":"1.0","type":"link"}