{"product_id":"cst-60153s","title":"CST,  60153S, NDUFS1 Antibody","description":"Polyclonal Antibody for studying NDUFS1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, NDUFS1 Antibody (CST #60153) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNDUFS1 Antibody recognizes endogenous levels of total NDUFS1 protein. This antibody can detect the carboxyl terminal 47 kDa fragment produced by caspase cleavage during apoptosis.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile286 of human NDUFS1 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nNDUFS1 (NADH dehydrogenase Fe-S protein 1) is a nuclear encoded structural subunit of NADH: ubiquinone oxidoreductase (complex 1) in the mitochondrial electron transport chain (1). Mutations in NDUFS1 and other complex 1 subunits leading to mitochondrial dysfunction are associated with a number of neurological disorders (2-5). High-fat diet associated with type 2 diabetes leads to decreased expression of complex 1 subunits, including NDUFS1 (6). NDUFS1 is the target of cleavage by apoptotic caspases contributing to the loss of mitochondrial transmembrane potential, compromised mitochondrial respiration, increased mitochondrial reactive oxygen species (ROS) production, and loss of lysosomal integrity (7,8). NDUFS1 can also be cleaved by the lymphocyte protease granzyme B (9). Studies have also found that MDM2, which has a canonical role as an inhibitor of the tumor suppressor p53, can also sequester NDUFS1 in the cytoplasm, leading to respiratory dysfunction and increases in ROS (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCI-75k; CI-75kD; complex I 75kDa subunit; Complex I-75kD; complex I, mitochondrial respiratory chain, 75-kD subunit; MC1DN5; MGC26839; mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase); NADH-coenzyme Q reductase; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; NADH:ubiquinone oxidoreductase core subunit S1; NDUFS1; NDUS1; PRO1304\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 75\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46801013735593,"sku":"60153S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-60153s","provider":"Iright","version":"1.0","type":"link"}