{"product_id":"cst-60312s","title":"CST,  60312S, CRBN (F4I7F) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CRBN. Validated for WB,WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CRBN (F4I7F) Rabbit Monoclonal Antibody (CST #60312) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nImmunofluorescence (Frozen): 1:100 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:100 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 95258 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCRBN (F4I7F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CRBN protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human CRBN protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCRBN\/Cereblon, a substrate receptor for the CRL4 E3 ubiquitin ligase, was originally identified for its role in autosomal recessive nonsyndromic mental retardation (1). CRBN's role in the regulation of the AMPK pathway has been linked to various human diseases, including cardiovascular disease and obesity, and its involvement in proteasomal degradation may be important in neuropsychiatric diseases (2). CRBN has also been shown to enhance the effects of immunomodulatory imide drugs (IMiDs) in multiple myeloma, and may be a predictive marker for therapy (2-4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncereblon; CRBN; DKFZp781K0715; MGC27358; MRT2; MRT2A; Protein cereblon; protein x 0001\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801014227113,"sku":"60312S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-60312s","provider":"Iright","version":"1.0","type":"link"}