{"product_id":"cst-60577t","title":"CST,  60577T, CD133 (A8N6N) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying CD133. Validated for Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, CD133 (A8N6N) Mouse Monoclonal Antibody (CST #60577) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Live): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD133 (A8N6N) Mouse Monoclonal Antibody recognizes endogenous levels of total CD133 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with cells overexpressing human CD133 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAC133; Antigen AC133; CD133; CORD12; hematopoietic stem cell antigen; hProminin; MCDR2; MSTP061; PROM1; prominin 1; Prominin-1; prominin-like 1; Prominin-like protein 1; PROML1; RP41; STGD4\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgG3","brand":"CST","offers":[{"title":"Default Title","offer_id":46800331178153,"sku":"60577T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-60577t","provider":"Iright","version":"1.0","type":"link"}