{"product_id":"cst-61060sf","title":"CST,  61060SF, Bcl-xL (54H6) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying BCL-xL. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, Bcl-xL (54H6) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #61060) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier-free version of product #2764. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN, or CUT\u0026amp;Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product #www.cellsignal.com\/products\/primary-antibodies\/bcl-xl-54h6-rabbit-mab\/2764?site-search-type=Products\u0026amp;N=4294956287\u0026amp;Ntt=bcl-xl+%2854h6%29\u0026amp;fromPage=plp\"\u0026gt;2764.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBcl-xL (54H6) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Bcl-xL protein. This antibody does not cross-react with other Bcl-xL proteins.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nBcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents, such as paclitaxel, vinblastine, and nocodazole (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nApoptosis regulator Bcl-X; B2CL1; Bcl-2-like protein 1; Bcl-X; bcl-xL; BCL-XL\/S; bcl-xS; BCL2 like 1; Bcl2-L-1; BCL2-like 1; BCL2L; BCL2L1; BCLX; BCLXL; BCLXS; DKFZp781P2092; PPP1R52; protein phosphatase 1, regulatory subunit 52\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801018912937,"sku":"61060SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-61060sf","provider":"Iright","version":"1.0","type":"link"}