{"product_id":"cst-61521s","title":"CST,  61521S, TRMT112 (F1A6F) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TRMT112. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TRMT112 (F1A6F) Rabbit Monoclonal Antibody (CST #61521) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTRMT112 (F1A6F) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRMT112 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human TRMT112 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMultifunctional methyltransferase subunit TRM112-like protein (TRMT112) is an evolutionarily conserved 15 kDa protein that interacts with and stabilizes various methyltransferases involved in tRNA, rRNA, and protein methylation. While most studies have been in yeast, TRMT112 has been shown to interact with human methyltransferases involved in translation and ribosome biogenesis, including TRMT11, ALKBH8, HEMK2a, WBSCR22, and METTL5 (1-5). TRMT112 is also widely expressed during embryogenesis (6). The TRMT11-TRMT112 complex deposits 2-methylguanosine at position 10 of tRNA, while ALKBH8-TRMT112 modifies a uridine at position 34 of the anti-codon loop (5,7) Additionally, TRMT112 plays a role in translation termination by interacting with N6AMT1, causing methylation of the release factor eRF1 to allow for stop codon recognition (8,9). TRMT112 also contributes to ribosome biogenesis by interacting with and stabilizing WBSCR22, an 18s rRNA methyltransferase, and METTL5, the 18s rRNA m6a-methyltransferase (3,4,10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHSPC152; HSPC170; HSPC170 protein; hTrm112; Multifunctional methyltransferase subunit TRM112-like protein; TR112; TRM112; TRM112-like protein; TRMT11-2; TRMT112; tRNA methyltransferase 11-2 homolog; tRNA methyltransferase 11-2 homolog (S. cerevisiae); tRNA methyltransferase 112 homolog; tRNA methyltransferase subunit 11-2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 15\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801022288041,"sku":"61521S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-61521s","provider":"Iright","version":"1.0","type":"link"}