{"product_id":"cst-61824s","title":"CST,  61824S, Tox\/Tox2 (E6G5O) Rabbit Monoclonal Antibody (Alexa Fluor® 594 Conjugate)","description":"Monoclonal Antibody for studying Tox2\/Tox. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Tox\/Tox2 (E6G5O) Rabbit Monoclonal Antibody (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 594 Conjugate) (CST #61824) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:200 - 1:400\nImmunofluorescence (Immunocytochemistry): 1:3200 - 1:6400\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTox\/Tox2 (E6G5O) Rabbit Monoclonal Antibody (Alexa Fluor\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln435 of human Tox protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2). Tox plays a key role in T cell development in the thymus during positive selection (3-5). A study in Tox-deficient mice also revealed a requirement for Tox in CD4 T cell and NK cell lineage development, including NKT cells, FoxP3+ T regulatory T cells, and lymphoid tissue-inducer (LTi) cells (6-8). Although Tox expression is primarily restricted to developing immune cells in normal tissues, Tox is induced by high antigen stimulation during chronic viral infection or cancer, regulating T cell persistence and exhaustion (9-12). Tox has also been shown to be aberrantly expressed in cutaneous T cell lymphomas (13-14).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC20orf100; dJ1108D11.2; dJ495O3.1; GCX-1; GCX1; granulosa cell HMG box 1; Granulosa cell HMG box protein 1; granulosa cell HMG-box protein 1; KIAA0808; MGC15880; thymocyte selection associated high mobility group box; thymocyte selection-associated high mobility group box; Thymocyte selection-associated high mobility group box protein TOX; Thymus high mobility group box protein TOX; TOX; TOX high mobility group box family member 2; TOX1; TOX2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801067901097,"sku":"61824S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-61824s","provider":"Iright","version":"1.0","type":"link"}