{"product_id":"cst-62115t","title":"CST,  62115T, DHCR7 (F7I5B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying DHCR7. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DHCR7 (F7I5B) Rabbit Monoclonal Antibody (CST #62115) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDHCR7 (F7I5B) Rabbit mAb recognizes endogenous levels of total DHCR7 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DHCR7 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\n7-dehydrocholesterol reductase (DHCR7) catalyzes the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol, the last step of cholesterol biosynthesis. Studies show that DHCR7 expression is downregulated in macrophages upon viral infection. DHCR7 deficiency activates the PI3K-AKT3 pathway. AKT3 phosphorylates interferon regulatory factor 3 (IRF3) at Ser385, leading to IRF3 activation and increased type I interferon (IFN-I) production in macrophages (1). In addition, DHCR7 has proferroptotic activity. Its substrate, 7-DHC, suppresses ferroptosis by inhibiting phospholipid peroxidation. Blocking 7-DHC biosynthesis enhances ferroptosis of cancer cells (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n7-dehydrocholesterol reductase; 7-DHC reductase; D7SR; delta-7-dehydrocholesterol reductase; Delta7-sterol reductase; DHCR7; Putative sterol reductase SR-2; SLOS; sterol delta-7-reductase; Sterol Delta(7)-reductase; Sterol reductase SR-2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801027072169,"sku":"62115T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-62115t","provider":"Iright","version":"1.0","type":"link"}