{"product_id":"cst-62586sf","title":"CST,  62586SF, FAP (E1V9V) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying FAP. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, FAP (E1V9V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #62586) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #66562. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 66562\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFAP (E1V9V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total FAP protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala497 of human FAP protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nFibroblast activation protein\/seprase (FAP) is a cell surface serine integral membrane protease with roles in tissue remodeling during development and wound healing, as well as in cancer and fibrotic disease. FAP has both dipeptidyl peptidase and endopeptidase activities, and cleaves at N-terminal Xaa-Pro sequences. FAP is highly expressed in tumor stroma, and contributes to migration and invasion of tumor cells, as well as that of endothelial cells, into the extracellular matrix. FAP is a useful marker of tumorigenic stroma and of cancer-associated fibroblasts (CAFs) and fibrosis (1,2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n170 kDa melanoma membrane-bound gelatinase; Antiplasmin-cleaving enzyme FAP, soluble form; APCE; Dipeptidyl peptidase FAP; DKFZp686G13158; DPPIV; FAP; FAPA; FAPalpha; Fibroblast activation protein alpha; fibroblast activation protein, alpha; fibroblast activation protein, alpha subunit; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Prolyl endopeptidase FAP; SEPR; Seprase; Serine integral membrane protease; SIMP; Surface-expressed protease\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 90\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801029759145,"sku":"62586SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-62586sf","provider":"Iright","version":"1.0","type":"link"}