{"product_id":"cst-62675s","title":"CST,  62675S, IFN-gamma (XMG1.2) Rat Monoclonal Antibody (PE Conjugate)","description":"Monoclonal Antibody for studying IFN-gamma mouse. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, IFN-gamma (XMG1.2) Rat Monoclonal Antibody (PE Conjugate) (CST #62675) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal flow cytometry results, we recommend 0.125 μg of antibody per test.\nFlow Cytometry (Fixed\/Permeabilized): 1:160\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIFN-gamma (XMG1.2) Rat Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total IFN-γ protein. This antibody conjugate detects an epitope within the intracellular domain.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.\n\u003cb\u003eBackground\u003c\/b\u003e\nIFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes cell-mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). Due to differences in the degree of glycosylation, there are three forms of IFN-γ, with approximate molecular weights of 25, 20, and 15.5 kDa by SDS-PAGE (5). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1\/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1\/IFNGR2) -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in or . IFN-γ also appears to have a role in atherosclerosis (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ngamma interferon; Ifg; IFN-g; IFN-gamma; Ifng; Interferon gamma; interferon, gamma\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rat IgG1 kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46801069047977,"sku":"62675S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-62675s","provider":"Iright","version":"1.0","type":"link"}