{"product_id":"cst-62823s","title":"CST,  62823S, SARS-CoV-2 EndoRNAse Antibody","description":"Polyclonal Antibody for studying endoRNAse-pp1ab SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 EndoRNAse Antibody (CST #62823) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSARS-CoV-2 EndoRNAse Antibody recognizes transfected levels of total SARS-CoV-2 endoRNAse protein.\nSpecies Reactivity: Virus\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of SARS-CoV-2 endoRNAse protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). SARS-CoV-2 Endonuclease, also called Nsp15, is translated from ORF1b. It is a uridine specific endoribonuclease that is thought to specifically cleave polyuridine RNA sequence at the 5' end of the template negative strand. Since polyuridine RNA can trigger the PAMP response, this action of the nuclease may block interferon production (3). It is also proposed to prevent accumulation of dsRNA intermediates and the formation of stress granules which are cell anti-viral responses (4). Together, these activities may prevent activation of host cell immune responses.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nendoRNAse; endoRNAse-pp1ab; NendoU; non-structural protein 15; nsp15; Nsp15 (6453-6798); SARS-CoV-2 nsp15; Uridylate-specific endoribonuclease\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: Vir\nSENSITIVITY: Transfected Only\nMW (kDa): 45\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800244211881,"sku":"62823S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-62823s","provider":"Iright","version":"1.0","type":"link"}