{"product_id":"cst-6301s","title":"CST,  6301S, SignalSilence® GSK-3 alpha\/beta siRNA","description":"siRNA for studying GSK3-alpha\/GSK3-beta in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nCST recommends transfection with 50 nM SignalSilence ® GSK-3 alpha\/beta siRNA 48 hours prior to cell lysis. See Protocol for transfection procedure.\n\u003cb\u003eStorage\u003c\/b\u003e\nGSK-3α\/β siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.\n\u003cb\u003eBackground\u003c\/b\u003e\nGlycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine\/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K\/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in and is a component of the Wnt signaling pathway required for and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Small Interfering RNA (siRNA) has been used to specifically silence GSK-3alpha and beta expression, causing an upregulation of beta-catenin protein levels (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nglycogen synthase kinase-3; GSK 3alpha; GSK 3beta; GSK-3alpha; GSK-3beta; gsk-3β; GSK3alpha; GSK3beta; gsk3α; gsk3β; RNAi; SignalSilence; siRNA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H","brand":"CST","offers":[{"title":"Default Title","offer_id":46800788783273,"sku":"6301S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-6301s","provider":"Iright","version":"1.0","type":"link"}