{"product_id":"cst-63335t","title":"CST,  63335T, Sphingosine-1-Phosphate Receptor 1 (E8U3O) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying EDG1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Sphingosine-1-Phosphate Receptor 1 (E8U3O) Rabbit Monoclonal Antibody (CST #63335) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:1600 - 1:3200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSphingosine-1-Phosphate Receptor 1 (E8U3O) Rabbit Monoclonal Antibody recognizes endogenous levels of total sphingosine-1-phosphate receptor 1 protein. Nonspecific nuclear background may be observed by immunofluorescence in some cell lines, especially with higher than recommended concentrations of antibody.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly366 of human sphingosine-1-phosphate receptor 1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSphingosine-1-phosphate receptor 1 (S1PR1, EDG1) is a G protein-coupled receptor (GPCR), one of five members (S1PR1-5) belonging to the sphingosine-1-phosphate receptor (S1PR) family (1,2). This family of GPCRs has been shown to exhibit broad and often overlapping expression patterns across a wide array of tissues. The cognate ligand for S1PRs is sphingosine-1-phosphate (S1P), a phosphorylated lipid signaling molecule derived from sphingosine. S1P\/S1PR binding can trigger the activation and\/or inhibition of various downstream signaling pathways, depending on context. Pathways identified downstream of S1P\/S1PR include PI3K\/Akt, PLC, Ras\/MAPK and PKA (3,4). S1P\/S1PR1 signaling pathways play important roles in many basic cellular functions during normal development, including chemotaxis, vascular integrity, and immune cell egress, but can also contribute to a variety of pathological conditions, including atherogenesis, multiple sclerosis, and tumorigenesis (5-9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1PR1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; sphingosine 1-phosphate receptor EDG1; sphingosine-1-phosphate receptor 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 40-50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801033363625,"sku":"63335T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-63335t","provider":"Iright","version":"1.0","type":"link"}