{"product_id":"cst-63454t","title":"CST,  63454T, PGAM5 (Long Isoform) (E8C3L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PGAM5. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PGAM5 (Long Isoform) (E8C3L) Rabbit Monoclonal Antibody (CST #63454) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPGAM5 (Long Isoform) (E8C3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total PGAM5 long isoform protein. This antibody specifically recognizes the PGAM5 long isoform (UniProt #Q96HS1-1) and does not recognize the PGAM5 short isoform (UniProt #Q96HS1-2).\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PGAM5 long isoform protein (UniProt #Q96HS1-1).\n\u003cb\u003eBackground\u003c\/b\u003e\nPhosphoglycerate mutase family 5 (PGAM5) is a mitochondrial serine\/threonine protein phosphatase (1,2). PGAM5 is required for necrosis induced by TNF-α or reactive oxygen species (2). In addition, PGAM5 dephosphorylates FUNDC1 to induce mitophagy under mitochondrial stresses (3). PGAM5 is also essential for RIPK1-mediated mitophagy activation during extracellular matrix (ECM) detachment of cancer cells (4). Furthermore, PGAM5 is cleaved by the protease presenilin-associated rhomboid-like protein (PARL) in response to mitochondrial stresses (such as induced by carbonyl cyanide -chlorophenylhydrazone (CCCP) treatment). The cleaved PGAM5, released from the mitochondrial membrane, dephosphorylates β-catenin in the cytosol leading to its stabilization. Increased levels of β-catenin stimulate mitochondrial biogenesis (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBcl-XL-binding protein v68; BXLBV68; MGC5352; PGAM family member 5, mitochondrial serine\/threonine protein phosphatase; PGAM family member 5, serine\/threonine protein phosphatase, mitochondrial; PGAM5; Phosphoglycerate mutase family member 5; Serine\/threonine-protein phosphatase PGAM5, mitochondrial\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 28, 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799830876329,"sku":"63454T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-63454t","provider":"Iright","version":"1.0","type":"link"}