{"product_id":"cst-6362s","title":"CST,  6362S, SignalSilence® Bcl-xL siRNA I","description":"siRNA for studying BCL-xL in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nCST recommends transfection with 100 nM SignalSilence ® Bcl-xL siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.\n\u003cb\u003eStorage\u003c\/b\u003e\nSignalSilence ® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.\n\u003cb\u003eBackground\u003c\/b\u003e\nBcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents, such as paclitaxel, vinblastine, and nocodazole (4,5).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H","brand":"CST","offers":[{"title":"Default Title","offer_id":46800789504169,"sku":"6362S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-6362s","provider":"Iright","version":"1.0","type":"link"}