{"product_id":"cst-6394s","title":"CST,  6394S, SignalSilence® SQSTM1\/p62 siRNA I","description":"siRNA for studying SQSTM1 in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nCST recommends transfection with 100 nM SignalSilence ® SQSTM1\/p62 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.\n\u003cb\u003eStorage\u003c\/b\u003e\nSignalSilence ® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.\n\u003cb\u003eBackground\u003c\/b\u003e\nSequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5) and independently found to interact with PKCζ (6,7). SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3\/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity.\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H","brand":"CST","offers":[{"title":"Default Title","offer_id":46800789536937,"sku":"6394S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-6394s","provider":"Iright","version":"1.0","type":"link"}