{"product_id":"cst-64436s","title":"CST,  64436S, Cleaved Gasdermin B (Lys229) (E1Y2E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Gasdermin B (Lys229) cleaved. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Gasdermin B (Lys229) (E1Y2E) Rabbit Monoclonal Antibody (CST #64436) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved Gasdermin B (Lys229) (E1Y2E) Rabbit Monoclonal Antibody recognizes transfected levels of Gasdermin B protein only when cleaved at Lys229.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys229 of human Gasdermin B protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe gasdermin family, which includes GSDMA, GSDMB, GSDMC, GSDMD, and GSDME, has been shown to play a role in inflammation and cell death. Gasdermin D has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11\/4\/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7). Gasdermin B (GSDMB) has been reported to be upregulated in several tumor types, and in breast cancer, has been associated with metastasis and poor prognosis (8,9). In addition, Gasdermin B has been associated with immune disorders, including asthma (10,11). Gasdermin B expression has also been found in the lung epithelium associated with asthma. Gasdermin B can also have a role in pyroptosis as it was found to activate caspase-4 and promote Gasdermin D cleavage (12). Cleavage and the pore-forming ability of Gasdermin B are induced by Granzyme A secreted by cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells (13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ngasdermin B; Gasdermin-B; Gasdermin-like protein; gasderminB-1; GSDMB; GSDMB iso4; GSDMB-1; GSDML; PP4052; PRO2521\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Transfected Only\nMW (kDa): 25\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799835365545,"sku":"64436S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-64436s","provider":"Iright","version":"1.0","type":"link"}