{"product_id":"cst-6451s","title":"CST,  6451S, SignalSilence® Rb siRNA I","description":"siRNA for studying Rb in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nCST recommends transfection with 50-100 nM SignalSilence ® Rb siRNA I 48 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.\n\u003cb\u003eStorage\u003c\/b\u003e\nSignalSilence ® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4\/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK\/cyclin complexes has been observed (6-8) and cyclin D1 is required for Ser780 phosphorylation (9). RNA interference has been used to silence Rb protein expression in prostate cancer cells resulting in increased Bcl-2 protein levels (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nP105-RB; PP105; PP110; RB-1; RB1; Retinoblastoma-associated protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H","brand":"CST","offers":[{"title":"Default Title","offer_id":46800790061225,"sku":"6451S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-6451s","provider":"Iright","version":"1.0","type":"link"}