{"product_id":"cst-64786s","title":"CST,  64786S, CD8 alpha (2.43) Rat Monoclonal Antibody (APC Conjugate)","description":"Monoclonal Antibody for studying CD8A mouse. Validated for Immunofluorescence (Frozen),Flow Cytometry (Fixed\/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD8 alpha (2.43) Rat Monoclonal Antibody (APC Conjugate) (CST #64786) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal flow cytometry results, we recommend 0.125 μg of antibody per test.\nImmunofluorescence (Frozen): 1:50 - 1:200\nFlow Cytometry (Fixed\/Permeabilized): 1:160\nFlow Cytometry (Live): 1:160\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen), Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD8 alpha (2.43) Rat Monoclonal Antibody (APC Conjugate) recognizes endogenous levels of total CD8α protein. This antibody conjugate detects an epitope within the extracellular domain.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.\n\u003cb\u003eBackground\u003c\/b\u003e\nCluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBB154331; CD8 antigen, alpha chain; Cd8a; Ly-; Ly-2; Ly-3; Ly-35; Ly-B; Lyt-; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rat IgG2b","brand":"CST","offers":[{"title":"Default Title","offer_id":46801073471657,"sku":"64786S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-64786s","provider":"Iright","version":"1.0","type":"link"}