{"product_id":"cst-6487t","title":"CST,  6487T, alpha-Actinin (D6F6) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ACTN1. Validated for Western Blotting,Fluorescent Western,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, alpha-Actinin (D6F6) Rabbit Monoclonal Antibody (CST #6487) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nFluorescent Western: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:100 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Fluorescent Western, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nalpha-Actinin (D6F6) Rabbit Monoclonal Antibody recognizes endogenous levels of total α-actinin protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe316 of human α-actinin-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nα-Actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. The α-actinin protein interacts with a large number of proteins involved in signaling to the cytoskeleton, including those involved in cellular adhesion, migration, and immune cell targeting (1). The interaction of α-actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). The cytoskeletal α-actinin isoforms 1 and 4 (ACTN1, ACTN4) are non-muscle proteins that are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia. The muscle isoforms 2 and 3 (ACTN2, ACTN3) localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nactinin 1 smooth muscle; actinin alpha 1; actinin, alpha 1; ACTN1; alpha-actinin; alpha-actinin 1; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; BDPLT15; epididymis secretory sperm binding protein; F-actin cross-linking protein; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799837233321,"sku":"6487T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-6487t","provider":"Iright","version":"1.0","type":"link"}