{"product_id":"cst-64991sf","title":"CST,  64991SF, Cyclin D1 (DCS6) Mouse Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying Cyclin D1. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Cyclin D1 (DCS6) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #64991) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCyclin D1 (DCS6) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total cyclin D1 protein. This antibody does not cross-react with cyclin D2 or D3.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant human cyclin D1 corresponding to residues 151-170.\n\u003cb\u003eBackground\u003c\/b\u003e\nActivity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip\/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D\/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D\/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1\/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4). Of the three D cyclins, aberrant expression of cyclin D1 has been associated with many forms of cancer including B cell lymphomas and can directly contribute to oncogenesis by various mechanisms including gene translocation or amplification (2). Cyclin D1 also plays a critical role in mammary tissue maturation (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB-cell CLL\/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; BCL1; CCND1; CycD1; cyclin D1; D11S287E; G1\/S-specific cyclin D1; G1\/S-specific cyclin-D1; PRAD1; PRAD1 oncogene; U21B31\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 36\nSource\/Isotype: Mouse IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46799837593769,"sku":"64991SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-64991sf","provider":"Iright","version":"1.0","type":"link"}