{"product_id":"cst-65130s","title":"CST,  65130S, HOXB7 (E3H4S) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HOXB7. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, HOXB7 (E3H4S) Rabbit Monoclonal Antibody (CST #65130) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHOXB7 (E3H4S) Rabbit Monoclonal Antibody recognizes endogenous levels of total HOXB7 protein. This antibody also detects a 20 kDa band of unknown origin.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human HOXB7 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHomeobox (HOX) proteins are homeodomain transcription factors (1). They bind to sequence-specific DNA motifs (C\/G)TAATTG and function as master transcriptional regulators, controlling the coordinated expression of genes involved in embryogenesis and tissue differentiation (1,2). HOXB7 belongs to the B group of HOX proteins and plays a dominant role in facilitating tumor progression in many solid tumors. Overexpression of HOXB7 is found in many types of tumors, including melanoma, ovarian, GI-tract, pancreatic ductal adenocarcinoma (PDAC), lung, gliomas, and breast cancers. Aberrant high expression of HOXB7 is associated with poor prognosis (3). The protein promotes tumor cell proliferation, angiogenesis, epithelial-mesenchymal transition, migration, and invasion, as well as drug resistance through regulation of multiple signaling pathways, such as MAPK, VEGF, FGF, TGF-β, and EGFR\/Her2 (4-8). HOXB7 binding to a DNA promoter requires its interaction with a cofactor protein such as PBX2 or CBP for transcriptional activation of downstream targets (9,10). Disruption of the HOXB7\/PBX complex formation by antagonist peptide HXR9 can inhibit HOXB7 function and is considered as a potential therapeutic value (11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHHO.C1; homeo box 2C; homeo box B7; homeo box c1 protein; homeobox B7; Homeobox protein HHO.C1; Homeobox protein Hox-2C; Homeobox protein Hox-B7; Hox-2.3; HOX2; HOX2C; HOXB7; HXB7\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 27\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799838937257,"sku":"65130S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-65130s","provider":"Iright","version":"1.0","type":"link"}