{"product_id":"cst-66006sf","title":"CST,  66006SF, ELAVL1\/HuR (D9W7E) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying ELAVL1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, ELAVL1\/HuR (D9W7E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #66006) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #12582. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12582\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nELAVL1\/HuR (D9W7E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ELAVL1\/HuR protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His201 of human ELAVL1\/HuR protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe ELAVL (embryonic lethal, abnormal vision and Drosophila-like) family of proteins includes ELAVL1\/HuR, ELAVL2\/HuB, ELAVL3\/HuC and ELAVL4\/HuD (1). ELAVL1\/HuR is ubiquitously expressed whereas expression of the other three members is neuronal-specific (1). ELAVL\/Hu proteins are highly conserved RNA-binding proteins (1). Besides three RNA recognition motifs, these proteins also contain nuclear localization signals that enable them to shuttle between nucleus and cytoplasm (2). Upon inhibition of transcription by actinomycin D, ELAVL1\/HuR relocates from nucleus to cytoplasm where it binds the AU-rich elements within 3' UTRs to stabilize mRNAs (3, 4). ELAVL1\/HuR is suggested to increase translation by binding to mRNAs (5,6). In addition, ELAVL1\/HuR interacts with microRNAs (miRNAs) (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); ELAV like RNA binding protein 1; ELAV-like protein 1; ELAV1; ELAVL1; embryonic lethal, abnormal vision, drosophila, homolog-like 1; Hu antigen R; Hu-antigen R; Hua; HUR; MelG\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799844278441,"sku":"66006SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-66006sf","provider":"Iright","version":"1.0","type":"link"}