{"product_id":"cst-66012s","title":"CST,  66012S, PLEKHM1 Antibody","description":"Polyclonal Antibody for studying PLEKHM1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, PLEKHM1 Antibody (CST #66012) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPLEKHM1 Antibody recognizes endogenous levels of total PLEKHM1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro455 of human PLEKHM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nPleckstrin homology domain-containing protein family member 1 (PLEKHM1) is a multivalent endolysosomal adaptor protein that contains an N-terminal RUN domain, two internal PH domains, and a C-terminal C1 domain\/zinc-finger-like motif. Research studies have shown that PLEKHM1 plays a role in bone resorption by regulating the acidification of endosomal vesicles of osteoclasts. Loss-of-function mutations in promote osteoclast failure and impair bone resorption, which leads to osteopetrosis (1-3). Recent studies have shown that PLEKHM1 serves as a nexus for different trafficking pathways of the endolysosomal network by serving as the platform for the recruitment of Rab7, HOPS complex, and LC3\/GABARAP family members. Indeed, loss of PLEKHM1 impairs the lysosomal degradation of cell-surface receptors and autophagic clearance of protein aggregates (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n162 kDa adapter protein; AP162; B2; KIAA0356; OPTA3; OPTB6; PH domain-containing family M member 1; PKHM1; pleckstrin homology and RUN domain containing M1; pleckstrin homology domain containing, family M (with RUN domain) member 1; Pleckstrin homology domain-containing family M member 1; PLEKHM1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 140\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799844245673,"sku":"66012S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-66012s","provider":"Iright","version":"1.0","type":"link"}