{"product_id":"cst-66973s","title":"CST,  66973S, CD11b\/ITGAM (E6E1M) Feline Chimeric Monoclonal Antibody","description":"Monoclonal Antibody for studying CD11b\/ITGAM mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, CD11b\/ITGAM (E6E1M) Feline Chimeric Monoclonal Antibody (CST #66973) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD11b\/ITGAM (E6E1M) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total CD11b\/ITGAM protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis recombinant chimeric antibody is engineered from CD11b\/ITGAM (E6E1M) Rabbit mAb #17800 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, CD11b\/ITGAM (E6E1M) Rabbit mAb #17800, is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse CD11b\/ITGAM protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCluster of differentiation molecule 11b (CD11b)\/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD11 antigen-like family member B; Cd11b; CD11B (p170); CD11b\/CD18; cell surface glycoprotein MAC-1 alpha subunit; Cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; CR3; CR3A; F730045J24Rik; integrin alpha M; Integrin alpha-M; ITAM; Itgam; Leukocyte adhesion receptor MO1; Ly-4; Ly-40; Mac-; Mac-1; Mac-1 alpha; Mac-1a; MAC1; macrophage antigen alpha\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Feline chimeric IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800297394345,"sku":"66973S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-66973s","provider":"Iright","version":"1.0","type":"link"}