{"product_id":"cst-67311t","title":"CST,  67311T, eIF2 beta (F2R7D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying eIF2-beta. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, eIF2 beta (F2R7D) Rabbit Monoclonal Antibody (CST #67311) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\neIF2 beta (F2R7D) Rabbit Monoclonal Antibody recognizes endogenous levels of eIF2β protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly323 of human eIF2β protein.\n\u003cb\u003eBackground\u003c\/b\u003e\neIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2β (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK\/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2β complex and inhibits the turnover of eIF2β. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). The eIF2β subunit plays a major role in various interactions of the eIF2 trimer with other proteins. eIF2β has been shown to directly interact with other eIF factors, including eIF5, eIF2B, and eIF3 (7-9). eIF2β has also been shown to directly bind mRNA through a C-terminal zinc-finger motif (10,11). Phosphorylation of eIF2β by CK2 promotes the guanine nucleotide exchange activity of eIF2β (12,13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp686L18198; eIF-2-beta; EIF2; eIF2-beta; EIF2B; EIF2beta; EIF2S2; eukaryotic initiation factor 2-beta; eukaryotic translation initiation factor 2 beta; Eukaryotic translation initiation factor 2 subunit 2; Eukaryotic translation initiation factor 2 subunit beta; eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa; IF2B; MGC8508; PPP1R67; protein phosphatase 1, regulatory subunit 67\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 48\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799849160873,"sku":"67311T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-67311t","provider":"Iright","version":"1.0","type":"link"}