{"product_id":"cst-67750s","title":"CST,  67750S, SARS-CoV-2 ORF7a Antibody","description":"Polyclonal Antibody for studying ORF7a protein SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 ORF7a Antibody (CST #67750) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSARS-CoV-2 ORF7a Antibody recognizes endogenous levels of total SARS-CoV-2 ORF7a protein.\nSpecies Reactivity: Virus\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val74 of SARS-CoV-2 ORF7a protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nSARS-CoV-2 ORF7a is a type-I transmembrane protein with an amino-terminal immunoglobulin (IgG)-like domain involved in immune modulation and a short cytosolic tail that contains an endoplasmic reticulum (ER) retention motif that mediates trafficking to the ER-Golgi intermediate complex (ERGIC) (1). Studies of ORF7a in SARS-CoV identified roles in apoptosis and cell cycle progression (2-4). Regulation of apoptosis has been attributed to an association with Bcl-xL, a key pro-survival Bcl-2 family member (5). Studies in both SARS-CoV and SARS-CoV-2 have shown that ORF7a can promote viral release through inhibition of BST2 (6,7). There are also reports that SARS-CoV-2 contributes to immune regulation as part of the viral life cycle. It can inhibit type I interferon signaling through inhibition of STAT2 phosphorylation (8). However, it can also lead to activation of NF-κB and induce the expression of inflammatory cytokines (9). ORF7a has also been shown to inhibit late stages of autophagy by reducing lysosomal acidity (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAccessory protein 7a; NS7A_SARS2; ORF7a protein; Protein 7a; Protein U122; Protein X4; SARS-CoV-2 accessory protein 7a; Severe acute respiratory syndrome coronavirus 2 ORF7a protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: Vir\nSENSITIVITY: Endogenous\nMW (kDa): 9-14\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800244539561,"sku":"67750S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-67750s","provider":"Iright","version":"1.0","type":"link"}