{"product_id":"cst-67824t","title":"CST,  67824T, ASC\/TMS1 (D2W8U) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ASC mouse. Validated for WB,IP,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, ASC\/TMS1 (D2W8U) Rabbit Monoclonal Antibody (CST #67824) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:100 - 1:400\nImmunofluorescence (Frozen): 1:400 - 1:1600\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 37953 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nASC\/TMS1 (D2W8U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ASC\/TMS1 protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant mouse ASC\/TMS1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTMS1 (target of methylation-induced silencing)\/ASC (apoptosis-associated speck-like protein containing a CARD), also referred to as PYCARD and CARD5, is a 22 kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD) (1-2). The gene was originally found to be aberrantly methylated and silenced in breast cancer cells (2), and has since been found to be silenced in a number of other cancers, including ovarian cancer (3), glioblastoma (4), melanoma (5), gastric cancer (6), lung cancer (7), and prostate cancer (8). Expression of ASC\/TMS1 can be induced by pro-apoptotic\/inflammatory stimuli (9). During apoptosis ASC\/TMS1 is redistributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis (10). ASC\/TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n9130417A21Rik; Apoptosis-associated speck-like protein containing a CARD; Asc; CARD5; mASC; OTTMUSP00000030782; Pycard; PYD and CARD domain containing; PYD and CARD domain-containing protein; TMS-; TMS-1; TNS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 22\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800110616745,"sku":"67824T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-67824t","provider":"Iright","version":"1.0","type":"link"}