{"product_id":"cst-67840t","title":"CST,  67840T, FUS\/TLS (E3O8I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying hnRNP P2. Validated for WB,IP,IF,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, FUS\/TLS (E3O8I) Rabbit Monoclonal Antibody (CST #67840) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor more information about the RBP-eCLIP service please visit Eclipsebio .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Frozen): 1:800 - 1:3200\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:3200\neCLIP: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier-free (BSA and azide free) version of this product see product # 48647 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFUS\/TLS (E3O8I) Rabbit Monoclonal Antibody recognizes endogenous levels of total FUS\/TLS protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln146 of human FUS\/TLS protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nFUS\/TLS (fused in sarcoma\/translocated in liposarcoma) was initially identified by investigators as a component of fusion proteins found in a variety of cancers, such as myxoid liposarcoma, acute myeloid leukemia, and Ewing's tumor (1). FUS\/TLS fusion with the DNA-binding domain of transcription activators, such as CHOP and ERG, leads to aberrant transcription of target genes that is thought by researchers to lead to tumor development (1-5). FUS\/TLS is involved in a wide range of RNA processing events, such as pre-mRNA splicing, mRNA transcription, and miRNA processing (1,6). In addition to its role in RNA metabolism, FUS\/TLS maintains genomic stability and co-regulates gene expression by interacting with various transcription factors such as nuclear receptors, YB-1, p65 subunit of NF-κB, TFIID, and RUNX2 (1,6,7). More recently, researchers have found several mutations of FUS\/TLS in ALS (amyotrophic lateral sclerosis) and FTLD (frontotemporal lobar degeneration) patients that causes cytoplasmic mislocalization of FUS\/TLS (6,8-12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n75 kDa DNA-pairing protein; ALS6; ETM4; FUS; FUS RNA binding protein; fus-like protein; FUS1; fused in sarcoma; fusion gene in myxoid liposarcoma; heterogeneous nuclear ribonucleoprotein P2; hnRNP P2; HNRNPP2; Oncogene FUS; Oncogene TLS; POMp75; RNA-binding protein FUS; TLS; Translocated in liposarcoma protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 70\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799852667049,"sku":"67840T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-67840t","provider":"Iright","version":"1.0","type":"link"}