{"product_id":"cst-67972t","title":"CST,  67972T, RBM14 (F8B9F) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying RBM14. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RBM14 (F8B9F) Rabbit Monoclonal Antibody (CST #67972) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRBM14 (F8B9F) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBM14 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly196 of human RBM14 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nRBM14, also known as coactivator activator (CoAA), is an RNA-binding protein (RBP) first characterized as a thyroid hormone receptor-binding protein (TRBP) coactivator, with two conserved RNA recognition motifs and a TRBP-interacting domain (1). RBM14 is a capable coactivator that can promote transcription at hormone-response elements and influence alternative splicing (1-3). The RBM14 transcript itself is alternatively spliced, including a shorter, dominant negative form lacking the TRBP-interacting domain, and is commonly referred to as CoAM. This shorter splice variant is upregulated during stem cell differentiation to help control splicing (4,5). The RBM14 protein plays major roles in regulating genomic integrity by influencing many processes, including differentiation, transcription, DNA damage, cell cycle, and condensate formation. RBM14 can function as a repressor by inhibiting the interaction of transcription factors with DNA (6). During non-homologous end joining, RBM14 can recruit XRCC4 and XLF to chromatin and release KU proteins, facilitating DNA repair (7,8). The role of RBM14 in DNA repair has been linked to radiation resistance in glioblastoma (9). In prostate cancer, RBM14 can drive prostate-specific antigen expression even under androgen deprivation (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCOAA; coactivator activator; DKFZp779J0927; MGC15912; MGC31756; Paraspeckle protein 2; PSP2; RBM14; RNA binding motif protein 14; RNA-binding motif protein 14; RNA-binding protein 14; RRM-containing coactivator activator\/modulator; SIP; Synaptotagmin-interacting protein; SYT interacting protein; SYT-interacting protein; SYTIP1; TMEM137; transmembrane protein 137\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 75\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799853420713,"sku":"67972T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-67972t","provider":"Iright","version":"1.0","type":"link"}