{"product_id":"cst-67988s","title":"CST,  67988S, SARS-CoV-2 RNA-dependent RNA Polymerase Antibody","description":"Polyclonal Antibody for studying RNA-dependent RNA polymerase-pp1ab SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 RNA-dependent RNA Polymerase Antibody (CST #67988) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSARS-CoV-2 RNA-dependent RNA Polymerase Antibody recognizes transfected levels of total SARS-CoV-2 RNA-dependent RNA polymerase protein.\nSpecies Reactivity: Virus\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala253 of SARS-CoV-2 RNA-dependent RNA polymerase protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). SARS-CoV-2 RNA-dependent RNA polymerase, RdRp, is a non-structural protein (Nsp12) translated from ORF1b. It is the key enzyme essential for transcription of viral RNA from RNA templates and is therefore critical to replication of the virus (2). The polymerase has a structure similar to all viral RNA RdRps, the so-called hand structure: the catalytic module consists of palm, fingers, and thumb domains, forming the encircled human right hand model. Some conservation exists between different RNA viruses in the palm and finger domains, but there are significant differences in other domains reflecting different catalytic modes of action (3). SARS-CoV-2 RdRp requires accessory proteins, Nsp7 and Nsp8, along with Nsp14 for transcriptional activity (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nnon-structural protein 12; nsp12; Nsp12 (4393-5324); Pol; RdRp; RNA-dependent RNA polymerase; RNA-dependent RNA polymerase-pp1ab; RNA-directed RNA polymerase; SARS-CoV-2 Nsp12\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: Vir\nSENSITIVITY: Transfected Only\nMW (kDa): 100\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800244277417,"sku":"67988S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-67988s","provider":"Iright","version":"1.0","type":"link"}