{"product_id":"cst-68179t","title":"CST,  68179T, Cyclin C (E6V4Z) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Cyclin C. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclin C (E6V4Z) Rabbit Monoclonal Antibody (CST #68179) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:1600 - 1:6400\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73204 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCyclin C (E6V4Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total cyclin C protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro263 of human cyclin C protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclin dependent kinases (CDKs) are regulated in part through binding to regulatory cyclins. While many cyclin\/CDK complexes regulate progression through the cell cycle, others, such as cyclin C\/CDK8, regulate transcription and other cellular functions. Cyclin C\/CDK8 forms a regulatory sub-complex with Med12 and Med13, which associates with the multi-subunit transcriptional coactivator Mediator Complex to modulate RNA polymerase II-dependent transcription. In addition to its role in transcription, cyclin C is important in the regulation of mitochondrial dynamics, apoptosis, and cell cycle progression (1). Cytosolic cyclin C induces mitochondrial fragmentation and apoptosis, and is required for activation of the Bcl2 family member, Bax (2). Cytoplasmic cyclin C also supports mitochondrial fission through dynamin-related protein 1 (Drp1) in response to oxidative stress (3). Cyclin C acts synergistically with PTEN to maintain levels of tumor suppressors p53 and p21 in a mouse model of thyroid cancer (4). In complex with CDK3, cyclin C regulates Rb-dependent exit from quiescence in human cells (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCCNC; CycC; cyclin C; Cyclin-C; hSRB11; SRB11; SRB11 homolog\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799854370985,"sku":"68179T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-68179t","provider":"Iright","version":"1.0","type":"link"}