{"product_id":"cst-68258t","title":"CST,  68258T, MHC Class II (LGII-612.14) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying HLA-DRB1\/HLA-DPB1. Validated for WB,WB,IP,IHC,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, MHC Class II (LGII-612.14) Mouse Monoclonal Antibody (CST #68258) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\nIHC Leica Bond: 1:800 - 1:3200\nImmunohistochemistry (Paraffin): 1:400 - 1:1600\nFlow Cytometry (Live): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 43816 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMHC Class II (LGII-612.14) Mouse Monoclonal Antibody exhibits strong reactivity with HLA-DRB and weak reactivity with HLA-DPB in cell lines transfected with constructs expressing Myc\/DDK-tagged HLA-DRB and HLA-DPB, respectively. Reactivity is not observed with HLA-DMB, HLA-DOB, and HLA-DQB in cell lines transfected with constructs expressing Myc\/DDK-tagged HLA-DMB, HLA-DOB, and HLA-DQB.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with cultured human B lymphoid cells treated with IFN-gamma.\n\u003cb\u003eBackground\u003c\/b\u003e\nMajor histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells, such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3). In the literature, this clone is reported to react with HLA-DR, HLA-DP, and HLA-DQ (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2B11; cell surface glycoprotein; clone P2-beta-3; Clone P2-beta-4; DPB1; DR-1; DR-16; DR-5; DR-8; DR1; DR16; DR5; DR8; DRB; DRB1; DRw10; DRw11; DRw8; FLJ75017; FLJ76359; HLA class II antigen beta chain; HLA class II histocompatibility antigen, DP beta 1 chain; HLA class II histocompatibility antigen, DP(W4) beta chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA class II histocompatibility antigen, DRB1-1 beta chain; HLA class II histocompatibility antigen, DRB1-10 beta chain; HLA class II histocompatibility antigen, DRB1-11 beta chain; HLA class II histocompatibility antigen, DRB1-16 beta chain; HLA class II histocompatibility antigen, DRB1-3 chain; HLA class II histocompatibility antigen, DRB1-8 beta chain; HLA-DP; HLA-DP histocompatibility type, beta-1 subunit; HLA-DP1B; HLA-DPB; HLA-DPB1; HLA-DR-beta 1; HLA-DR1B; HLA-DRB; HLA-DRB1; human leucocyte antigen DRB1; Human leukocyte antigen DRB1; leucocyte antigen DR beta 1 chain; leucocyte antigen DRB1; lymphocyte antigen DRB1; major histocompatibility complex, class II, DP beta 1; major histocompatibility complex, class II, DR beta 1; major histocompatibility complex, class II, DR beta 1 precursor; MHC class I antigen DRB1; MHC class II antigen DPB1; MHC class II antigen DRB1; MHC class II antigen HLA-DR13; MHC class II HLA-DP-beta-1; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC HLA DPB1; SS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 25-35, 50-65\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46799854534825,"sku":"68258T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-68258t","provider":"Iright","version":"1.0","type":"link"}