{"product_id":"cst-68344s","title":"CST,  68344S, SARS-CoV-1\/2 Nucleocapsid Protein (1C7C7) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying Nucleocapsid Protein SARS-CoV-2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SARS-CoV-1\/2 Nucleocapsid Protein (1C7C7) Mouse Monoclonal Antibody (CST #68344) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSARS-CoV-1\/2 Nucleocapsid Protein (1C7C7) Mouse Monoclonal Antibody recognizes endogenous levels of total nucleocapsid (N) protein from SARS-CoV-1 and SARS-CoV-2.\nSpecies Reactivity: Virus\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein corresponding to SARS-CoV-1 nucleocapsid (N) protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). The nucleocapsid (N) protein is a phosphoprotein that plays a critical role in viral assembly (3,4). It binds to the viral genomic RNA, enabling its packaging into the ribonucleoprotein complex (RNP), and is the most abundant protein in the virion (5). Evidence from studies in SARS-CoV-2 and other coronaviruses suggest functions for nucleocapsid (N) protein beyond viral RNA packaging, including roles in viral replication (6) and suppressing host immune responses to viral infection (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2019-nCoV nucleocapsid protein; NC; NCAP_SARS2; nucleocapsid phosphoprotein; Nucleocapsid protein; Nucleoprotein; Protein N; SARS-CoV-2 nucleocapsid; Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: Vir\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSource\/Isotype: Mouse IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46800244441257,"sku":"68344S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-68344s","provider":"Iright","version":"1.0","type":"link"}