{"product_id":"cst-68621w2","title":"CST,  68621W2, Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (SignalFlex™ Pacific Blue™ Conjugate)","description":"Monoclonal Antibody for studying Stat4 (Tyr693) phosphate. Highly specific and rigorously validated in-house, Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (SignalFlex\u003csup\u003e™\u003c\/sup\u003e Pacific Blue\u003csup\u003e™\u003c\/sup\u003e Conjugate) (CST #68621) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions\/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Stat4 (Tyr693) (D2E4) Rabbit mAb (SignalFlex™ Pacific Blue™ Conjugate) recognizes endogenous levels of Stat4 protein only when phosphorylated at Tyr693.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr693 of human Stat4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors, and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins, resulting in their nuclear translocation, binding to specific DNA elements, and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined, in part, by the tissue-specific expression of different cytokine receptors, Jaks, and Stats, as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus, and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Activation of Stat4 is associated with phosphorylation at Tyr693 (9). Stat4 is activated in response to IL-2 in natural killer (NK) cells, but not in T cells (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nSignal transducer and activator of transcription 4; signal transducer and activator of transcription 4 variant 3; SLEB11; STAT4\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800512614569,"sku":"68621W2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-68621w2","provider":"Iright","version":"1.0","type":"link"}