{"product_id":"cst-6915t","title":"CST,  6915T, Miwi (D92B7) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Miwi mouse. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Miwi (D92B7) Rabbit Monoclonal Antibody (CST #6915) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:800 - 1:3200\nImmunofluorescence (Frozen): 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMiwi (D92B7) Rabbit Monoclonal Antibody recognizes endogenous levels of total Miwi protein. Non-specific staining was observed in a subset of mouse immune cells by immunohistochemistry.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly81 of mouse Miwi protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including , and (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nMGC150073; Miwi; piwi like homolog 1; piwi-like homolog 1; piwi-like homolog 1 (Drosophila); Piwi-like protein 1; piwi-like RNA-mediated gene silencing 1; Piwil1; PIWL1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 90\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799858761897,"sku":"6915T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-6915t","provider":"Iright","version":"1.0","type":"link"}