{"product_id":"cst-69238s","title":"CST,  69238S, Phospho-HDAC2 (Ser394) (E8O2Z) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HDAC2 (Ser394) phosphate. Validated for WB,IP,F,C\u0026amp;R,C\u0026amp;T. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-HDAC2 (Ser394) (E8O2Z) Rabbit Monoclonal Antibody (CST #69238) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThe CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 . The CUT\u0026amp;Tag dilution was determined using CUT\u0026amp;Tag Assay Kit # 77552 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:800\nCUT\u0026amp;RUN: 1:50\nCUT\u0026amp;Tag: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed\/Permeabilized), CUT\u0026amp;RUN, CUT\u0026amp;Tag\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-HDAC2 (Ser394) (E8O2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of HDAC2 protein only when phosphorylated at Ser394.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phophopeptide corresponding to residues surrounding Ser394 of human HDAC2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAcetylation of the histone tail causes chromatin to adopt an \"open\" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a \"closed\" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). Ser394 is phosphorylated by CK2, which promotes enzymatic activity and regulates complex formation (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHD2; HDAC 2; HDAC2; Histone deacetylase 2; KDAC2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1-associated factor 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 62\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799859351721,"sku":"69238S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-69238s","provider":"Iright","version":"1.0","type":"link"}