{"product_id":"cst-69375s","title":"CST,  69375S, NFI-A (E6Z1R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NFI-A. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, NFI-A (E6Z1R) Rabbit Monoclonal Antibody (CST #69375) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNFI-A (E6Z1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total NFI-A protein. Based on sequence homology, this antibody is not predicted to cross-react with other NFI family members.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro430 of human NFI-A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNFI-A belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). NFI-A activates cell-type-specific enhancers and facilitates the binding of PPARγ, the master transcriptional regulator of adipogenesis, to control the brown fat gene program (4). Additionally, NFI-A represses the expression of , the master transcriptional regulator of myogenesis, thus actively suppressing the myogenic gene program (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBRMUTD; CCAAT-box-binding transcription factor; CTF; DKFZp434L0422; DKFZp686J23256; FLJ39164; KIAA1439; NF-I\/A; NF1-A; NFI-A; NFI-L; NFIA; Nuclear factor 1 A-type; Nuclear factor 1\/A; nuclear factor I A; Nuclear factor I\/A; TGGCA-binding protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 70\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799860465833,"sku":"69375S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-69375s","provider":"Iright","version":"1.0","type":"link"}