{"product_id":"cst-69542s","title":"CST,  69542S, DNAJC9 (F4U7W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying DNAJC9. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DNAJC9 (F4U7W) Rabbit Monoclonal Antibody (CST #69542) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDNAJC9 (F4U7W) Rabbit mAb recognizes endogenous levels of total DNAJC9 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala236 of human DNAJC9 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nDnaJ homolog subfamily C member 9 (DNAJC9) serves a dual role as both a histone chaperone and a heat shock co-chaperone. As a histone chaperone, DNAJC9 forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers, potentially assisting MCM2 in recognizing these heterodimers and facilitating their assembly into nucleosomes. DNAJC9 interacts with HSP70, activating its ATPase activity (1). Overexpression of CENP-A, a centromeric histone H3 variant, is frequently observed in cancer. Its mislocalization to noncentromeric regions promotes chromosomal instability (CIN), a hallmark of cancer. DNAJC9 plays a crucial role in preventing the mislocalization of centromeric CENP-A, thereby mitigating chromosome instability (2). DNAJC9 expression is a potential prognostic biomarker in basal-like and luminal A breast cancer (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDnaJ (Hsp40) homolog, subfamily C, member 9; DnaJ heat shock protein family (Hsp40) member C9; DnaJ homolog subfamily C member 9; DnaJ protein SB73; DNAJC9; DNJC9; HDJC9; JDD1; KIAA0974; SB73\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799862464681,"sku":"69542S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-69542s","provider":"Iright","version":"1.0","type":"link"}