{"product_id":"cst-6961t","title":"CST,  6961T, Toll-like Receptor 3 (D10F10) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TLR3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Toll-like Receptor 3 (D10F10) Rabbit Monoclonal Antibody (CST #6961) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nToll-like Receptor 3 (D10F10) Rabbit Monoclonal Antibody recognizes endogenous levels of total TLR3 protein. A band is detected at 75 kDa in some cell lines\/tissues which is of unknown origin.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val495 of human TLR3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMembers of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in , play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll\/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adapter proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adapter-like\/TIR-associated protein (MAL\/TIRAP), TIR domain-containing adapter-inducing IFN-β (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm. TLR3 functions as a receptor for double-stranded (ds)RNA typically associated with viral infection (4). It was originally shown to be specifically expressed in dendritic cells of the leukocyte family (5). TLR3 has also been found in placenta and lung, and can be induced by LPS in a variety of tissues (4,6). TLR3 is predominantly localized to early endosomes (7,8). Binding of dsRNA, or the analog polyinosine-polycytidylic acid (pIpC), to TLR3 triggers activation of transcription factors NF-κB and IRF3 through the adaptor protein TICAM-1\/TRIF (9,10). TRIF associates with members of the TRAF family and with RIP that combine to activate NF-κB and IRF3 (11-13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD283; IIAE2; TLR3; toll like receptor 3; Toll-like receptor 3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 115-130\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799862890665,"sku":"6961T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-6961t","provider":"Iright","version":"1.0","type":"link"}