{"product_id":"cst-69625t","title":"CST,  69625T, Mis18-alpha (D3N2X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MIS18A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Mis18-alpha (D3N2X) Rabbit Monoclonal Antibody (CST #69625) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMis18-alpha (D3N2X) Rabbit Monoclonal Antibody recognizes endogenous levels of total Mis18-α protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg41 of human Mis18-α protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCENP-A, also known as the chromatin-associated protein CSE4 (capping-enzyme suppressor 4-p), is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin. The inherited localization of the centromere is specified by CENP-A (1). CENP-A deposition to the correct chromosomal location in early G1 phase is regulated by the Mis18 complex, which consists of Mis18-alpha, Mis18-beta, Mis18BP1, RbAp48 and RbAp46 (2). Mis18-alpha deficiency in mice results in inappropriate localization of CENP-A, as well as DNA methylation defects (3). Localization of the Mis18 complex to centromeres is regulated by the mitotic kinase Plk1 (polo-like kinase 1) (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB28; C21orf45; C21orf46; chromosome 21 open reading frame 45; FAPP1-associated protein 1; FASP1; hMis18alpha; MIS18 kinetochore protein A; MIS18 kinetochore protein homolog A; MIS18A; MIS18alpha; MS18A; Protein Mis18-alpha\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 26\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799863185577,"sku":"69625T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-69625t","provider":"Iright","version":"1.0","type":"link"}