{"product_id":"cst-6975s","title":"CST,  6975S, Rab8A (D22D8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying RAB8. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Rab8A (D22D8) Rabbit Monoclonal Antibody (CST #6975) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRab8A (D22D8) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rab8A protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys175 of human Rab8A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring (1). Studies show that Rab8 is localized to the trans Golgi network (TGN), basolateral membrane, and vesicular structures where it helps regulate target protein transport between TGN and the basolateral membrane (1-3). Overexpression studies and mutation analysis of Rab8 and its associated Rab8GEF indicate additional roles in actin and microtubule remodeling during polarized membrane transport and membrane protrusion formation (4-6). Rab8 associates with myosin Vb and is required for translocation of GLUT4 following insulin stimulation in muscle (7,8). Control of target protein vesicle transport by Rab8 also regulates MT1-MMP activity during extracellular matrix formation and JRAB\/MICAL-L2 at tight junction formation (9,10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nMEL; mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; mel transforming oncogene (RAB8 homolog); Oncogene c-mel; RAB8; RAB8A; RAB8A, member RAS oncogene family; ras-associated protein RAB8; Ras-related protein Rab-8A\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 23\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799863906473,"sku":"6975S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-6975s","provider":"Iright","version":"1.0","type":"link"}