{"product_id":"cst-6976t","title":"CST,  6976T, PAR2 (D61D5) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PAR-2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PAR2 (D61D5) Rabbit Monoclonal Antibody (CST #6976) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPAR2 (D61D5) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAR2 protein. The major band detected runs at 32 kDa, the higher molecular weight smear is due to glycosylation.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly44 of human PAR2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPAR2 (F2RL1) belongs to the PAR (Protease-activated Receptor) family of G protein-coupled receptors. These membrane receptors are activated through N-terminal cleavage of the receptor by a serine protease such as thrombin, trypsin, or matrix metalloproteinases (1,2). This cleavage exposes the ‘tethered-ligand' fragment of the receptor, which binds to a second extracellular loop of the receptor and leads to receptor activation. PAR2 is specifically activated by trypsin or trypsin-like proteases. Activated PAR2 stimulates phosphoinositide hydrolysis and calcium mobilization, interacts with β-arrestin, and eventually leads to ERK activation (3). PAR2 expression and activation are mainly associated with inflammatory diseases (3), but may also play a role in cancer development (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncoagulation factor II (thrombin) receptor-like 1; Coagulation factor II receptor-like 1; F2R like trypsin receptor 1; F2RL1; G protein-coupled receptor-11; G-protein coupled receptor 11; GPR11; PAR-2; PAR2; protease-activated receptor 2; Proteinase-activated receptor 2; Proteinase-activated receptor 2, alternate cleaved 1; Proteinase-activated receptor 2, alternate cleaved 2; proteinase-activated receptor-2; Thrombin receptor-like 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 32\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799864103081,"sku":"6976T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-6976t","provider":"Iright","version":"1.0","type":"link"}