{"product_id":"cst-69926t","title":"CST,  69926T, MMP-13 (E4W3T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MMP13. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MMP-13 (E4W3T) Rabbit Monoclonal Antibody (CST #69926) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMMP-13 (E4W3T) Rabbit Monoclonal Antibody recognizes endogenous levels of total human MMP13 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human MMP13 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMMP-13 (collagenase 3) belongs to the matrix metalloproteinase (MMP) superfamily of enzymes that targets many extracellular proteins, including other proteases, growth factors, cell surface receptors, and adhesion molecules (1,2). MMP-13 is a member of a subgroup of collagenases (including MMP-1, MMP-8, and MMP-18) that play an even more important function targeting fibrillar collagen. MMP-13 is synthesized as a latent proenzyme, and proteolytic removal of the inhibitory propeptide domain is required for enzyme activation. MMP-13 protein levels are regulated at the transcriptional level via specific transcription factors and promoter DNA methylation (3,4). MMP-13 preferentially cleaves Type II collagen, and research studies have shown that aberrant upregulation of MMP-13 activity can lead to cartilage loss and osteoarthritis (5,6). In addition, MMP-13 has been shown to promote cancer development, in part through enhancing tumor angiogenesis and metastases (7-9), suggesting that collagenase activity may serve as a useful marker of tumor progression (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCLG3; Collagenase 3; MANDP1; matrix metallopeptidase 13; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MDST; MMP-13; MMP13\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 60\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799865544873,"sku":"69926T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-69926t","provider":"Iright","version":"1.0","type":"link"}