{"product_id":"cst-70310sf","title":"CST,  70310SF, Galectin-3\/LGALS3 (D4I2R) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying galectin-3. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Galectin-3\/LGALS3 (D4I2R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #70310) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #87985. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 87985\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGalectin-3\/LGALS3 (D4I2R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Galectin-3\/LGALS3 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Galectin-3\/LGALS3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGalectins are a family of β-galactose binding proteins that are characterized by an affinity for poly-N-acetyllactosamine-enriched glycoconjugates and a carbohydrate-binding site (1,2). Members of the galectin family have been implicated in a variety of biological functions, including cell adhesion (3), growth regulation (4), cytokine production (5), T cell apoptosis (6), and immune responses (7). Galectin-3\/LGALS3 is involved in several diverse biological functions. Galectin-3\/LGALS3 binds IgE (8). Galectin-3\/LGALS3 is an unusual protein in that it can be found both extracellularly and intracellularly. Intracellularly, Galectin-3\/LGALS3 can localize to the cytoplasm, nucleus, or both, depending on cell type and experimental conditions. Nuclear Galectin-3\/LGALS3 has been identified as a pre-mRNA splicing factor (9). Galectin-3\/LGALS3 production has been shown to increase during inflammation and in obesity, and the protein itself can have an inflammatory effect under certain conditions (10). Galectin-3\/LGALS3 forms a complex with α3, β1 integrin to act as a surface receptor on endothelial cells for the NG2 proteoglycan, which triggers cell motility and angiogenesis (11). In addition to these functions, Galectin-3\/LGALS3 is also a required factor for the terminal differentiation of epithelial cells (12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP35; epididymis secretory sperm binding protein; Gal-3; GAL3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; galectin 3; Galectin-3; GALIG; IgE-binding protein; L-31; L31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LEG3; LGALS2; LGALS3; Mac-2 antigen; MAC2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799867183273,"sku":"70310SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-70310sf","provider":"Iright","version":"1.0","type":"link"}