{"product_id":"cst-70667s","title":"CST,  70667S, Cleaved TGF-beta1 (Ala279)\/TGF-beta3 (Ala301) (E5L1D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TGFB2 (Ala303) cleaved\/TGFB1 (Ala279) cleaved\/TGFB3 (Ala301) cleaved. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved TGF-beta1 (Ala279)\/TGF-beta3 (Ala301) (E5L1D) Rabbit Monoclonal Antibody (CST #70667) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved TGF-beta1 (Ala279)\/TGF-beta3 (Ala301) (E5L1D) Rabbit Monoclonal Antibody recognizes endogenous levels of TGF-β1 and TGF-β3 proteins only when cleaved at Ala279 and Ala301, respectively. This antibody does not cross-react with full-length TGF-β1, TGF-β2, or TGF-β3, nor does it react with cleaved TGF-β2. Upper bands present in human platelet and K-562 lanes are non-specific.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Ala279 and Ala301 of human TGF-β1 and TGF-β3 proteins, respectively.\n\u003cb\u003eBackground\u003c\/b\u003e\nTransforming growth factor-β (TGF-β) proteins belong to the TGF-β superfamily of cytokines that play a critical role in regulating cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-3). TGF-β ligands elicit signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII) TGF-β receptors. Type I and type II receptors are serine\/threonine kinases that form a heteromeric complex following ligand binding to the type II receptor. In response to ligand binding, the type II receptors form a stable complex with the type I receptors, triggering phosphorylation and activation of the type I receptor (4). This results in the recruitment of receptor-mediated SMADs (SMAD2, SMAD3), which are phosphorylated by the type I kinase in an SSXS domain in the C-terminus. This leads to recruitment of the co-SMAD (SMAD4), and subsequent translocation of this heteromeric SMAD complex to the nucleus, where it regulates transcription of target genes (5-7). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain. Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex (8). There are three TGF-β family members, designated TGF-β1, TGF-β2, and TGF-β3, which are encoded by distinct genes and are expressed in a tissue-specific manner (10). TGF-β proteins are synthesized as precursor proteins that are cleaved and reassembled in association with other proteins to form latent complexes. Activation occurs by proteolytic release of TGF-β monomers, which dimerize to form the mature TGF-β ligands.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nARVD; ARVD1; BSC-1 cell growth inhibitor; CED; Cetermin; DPD1; FLJ16571; G-TSF; Glioblastoma-derived T-cell suppressor factor; IBDIMDE; LAP; Latency-associated peptide; LDS4; LDS5; MGC116892; Polyergin; prepro-transforming growth factor beta-1; prepro-transforming growth factor beta-2; prepro-transforming growth factor beta-3; RNHF; TGF beta; TGF-beta 1 protein; TGF-beta-1; TGF-beta-2; TGF-beta-3; TGF-beta1; TGF-beta2; TGF-beta3; TGFB; TGFB1; TGFB2; TGFB3; TGFbeta; transforming growth factor beta 1; transforming growth factor beta 2; transforming growth factor beta 3; Transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; Transforming growth factor beta-2; Transforming growth factor beta-2 proprotein; Transforming growth factor beta-3; Transforming growth factor beta-3 proprotein; transforming growth factor beta1; transforming growth factor, beta 1; transforming growth factor, beta 2; transforming growth factor, beta 3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 12\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799869214889,"sku":"70667S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-70667s","provider":"Iright","version":"1.0","type":"link"}