{"product_id":"cst-71103sf","title":"CST,  71103SF, EOMES (D8D1R) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying EOMES. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, EOMES (D8D1R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #71103) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #81493. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 81493\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nEOMES (D8D1R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total EOMES protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro180 of human EOMES protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe T-box family of transcription factors is named for their shared homology with the DNA binding domain of the mouse brachyury (T) gene product. Members of this family bind DNA and are capable of transcriptional activation. They also have evolutionarily conserved expression patterns and roles in embryonic development, primarily mesoderm development (1). EOMES, or Tbr2 (T-box brain 2), is a master regulator of mesoderm formation that is also essential for trophoblast formation, gastrulation, neurogenesis, and the differentiation of certain T cell subsets. Embryos from EOMES knockout mice die soon after implantation due to their inability to develop a trophoblast (2,3). Conditional neural knockout mice show defects in development of a specific population of neural progenitors known as intermediate-stage progenitor cells (IPCs) that give rise only to neurons (4,5). These cells are formed from the radial glia in the ventricular and sub-ventricular zones of the cortex. Expression of EOMES increases as cells develop from radial glia to IPCs and then decreases as IPCs progress to neurons. Recent evidence suggests that EOMES and IPCs may also play a role in neurogenesis in the adult hippocampal SGZ (5). EOMES is also a key transcription factor for memory T cells and for full effector differentiation of CD8 T cells (6). Expression of EOMES is induced in CD8 T cells following viral infection and bacterial infection where sufficient IL-12 has been produced to elicit acute host cell response (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nEOMES; eomesodermin; Eomesodermin homolog; eomesodermin homolog (Xenopus laevis); t box, brain, 2; T-box brain protein 2; T-brain-2; TBR-2; TBR2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 75, 85\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799871606953,"sku":"71103SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-71103sf","provider":"Iright","version":"1.0","type":"link"}